The Matrix-Chaperone: Ambient Temperature Biospecimen Collection, Transport, and Banking for Simplified Animal Disease Screening

Principal Investigator(s): , ,

JWL_3819

This project is focused on development and testing of novel technology for the collection, preservation, and transport of biological samples at ambient temperature. The first phase of the project focused on lead compound identification for inactivating experimental samples in the “Matrix Chaperone” format. In phase II, the project was expanded to fine-tune and validate these tools using avian samples collected in the field using the sample collection devices developed during phase I. Validation was performed via collection and analysis of avian influenza virus and Newcastle disease virus from tracheal and cloacal swabs and avian blood draws, and analyzed via standard rt-PCR, virus isolation, and immunoassay methods, comparing samples placed in standard transport media to samples collected via Matrix Chaperone elastomers and swabs.

Outcomes and impacts

This project demonstrated that the Matrix Chaperone technology can preserve protein, nucleic acid, and live virus samples in a dry-state at ambient temperature for up to seven days. Eliminating cold chain requirements will simplify shipping of samples to diagnostic laboratories during a disease outbreak and has the potential to be especially impactful in the developing world where sample refrigeration is not always possible.

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