Diagnostic Tools

Development of Monoclonal Antibodies to Foot and Mouth Disease virus Structural Proteins

The goal of this project is to generate high-quality, monoclonal antibodies to support Foot and Mouth Disease (FMD) diagnosis and research. Monoclonal antibodies are a necessary component for many assays, including ones that are currently used and ones in development. This project will deliver novel monoclonal antibodies, including pan-viral reagents for use across all seven FMD virus serotypes. Learn more.

Universal Sample Preparation Tool for Bulk Milk Tank Monitoring

The purpose of this effort is to develop a sample collection, storage, and enrichment platform for the detection of viruses from large volume bulk milk tank samples. This project uses magnetic Nanotrap particle technology to capture and enrich the FMDv surrogate, Bovine Rhinovirus, from milk samples followed by qRT-PCR for detection. Learn more.

Agricultural Screening Tools

Based on recommendations from a series of workshops to coordinate and enhance the agro-security enterprise, the Institute for Infectious Animal Diseases (IIAD) proposed and was awarded a number of research efforts whose outcomes can be integrated with minimal disruption into daily business practices to help establish the “proof of negative” status essential to maintain business continuity. These research efforts will facilitate rapid sampling, testing and reporting of results to promote efficient and effective incident management. Learn more.

Optimization and Validation of a Real-Time RT-PCR Assay For Rapid Detection of Foot and Mouth Disease virus for Use in Bulk Tank Milk Samples

Objectives of this study are to define, optimize, and standardize RNA extraction and polymerase chain reaction (PCR)-mediated detection of Foot and Mouth Disease virus (FMDv) from bulk tank milk samples and obtain data suitable for development of a national surveillance plan for screening milk for FMDv. Surveillance after bulk-tank milk provides a pooled sample to determine herd/premises status during an FMDv outbreak. Demonstration of negative status supports product movement under the Security Milk Supply Plan for business continuity.  Learn more.

Development of a Mutiplex RT-qPCR Assay for Surveillance of Foreign Animal Diseases During Routine Testing of Oral Fluid Samples

This project developed a test that supports a comprehensive swine surveillance program for endemic disease testing with the multiplexed ability to test for foreign animal diseases (FAD) using the same pooled sample of oral fluids. The successful execution of this project will enable an economic and rapid method for FAD surveillance and testing, conserving reagents, which may be in short supply during an outbreak.  Learn more.

Development of a Prototype, Performance and Evaluation, and Validation and Transition of a 3ABC Foot and Mouth Disease virus Competitive Enzyme-Linked Immunosorbent Assay Kit 

The goal of these programs is to have a Foot and Mouth Disease (FMD) diagnostic test that is manufactured in the United States that can also be used as a Differentiation Infected from Vaccinated Animals (DIVA) test to differentiate cattle vaccinated with next generation FMD vaccines. The current scope of these programs supports transition of a “research use” kit to the U.S. Department of Agriculture (USDA) National Animal Health Laboratory Network laboratories. The ultimate goal is to have a USDA-licensed diagnostic kit.

Results to date indicate the kit performs better (improved diagnostic sensitivity and specificity) than other commercial tests and provides results in hours rather than days. The ability to produce and distribute a licensed DIVA diagnostic on the U.S. mainland will improve the response time and capacity, if there is an outbreak of this disease, enabling a more rapid recovery. Learn more.

The Matrix-Chaperone: Ambient Temperature Biospecimen Collection, Transport, and Banking for Simplified Animal Disease Screening

This project will develop and validate a novel field collection technology for the shipping and preservation of biological samples without the need for refrigeration or additional containment. Eliminating cold chain requirements will simplify sample shipping to diagnostic laboratories during a disease outbreak and has the potential to be especially impactful in the developing world where refrigeration is not always possible. Learn more.